U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection.
نویسندگان
چکیده
منابع مشابه
Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection.
Intron definition and splice site selection occur at an early stage during assembly of the spliceosome, the complex mediating pre-mRNA splicing. Association of U1 snRNP with the pre-mRNA is required for these early steps. We report here that the yeast U1 snRNP-specific protein Nam8p is a component of the commitment complexes, the first stable complexes assembled on pre-mRNA. In vitro and in viv...
متن کاملThe U1 snRNP-associated factor Luc7p affects 5′ splice site selection in yeast and human
yLuc7p is an essential subunit of the yeast U1 snRNP and contains two putative zinc fingers. Using RNA-protein cross-linking and directed site-specific proteolysis (DSSP), we have established that the N-terminal zinc finger of yLuc7p contacts the pre-mRNA in the 5' exon in a region close to the cap. Modifying the pre-mRNA sequence in the region contacted by yLuc7p affects splicing in a yLuc7p-d...
متن کاملThe U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex.
Recognition of the 5' splice site is an important step in mRNA splicing. To examine whether U1 approaches the 5' splice site as a solitary snRNP or as part of a multi-snRNP complex, we used a simplified in vitro system in which a short RNA containing the 5' splice site sequence served as a substrate in a binding reaction. This system allowed us to study the interactions of the snRNPs with the 5...
متن کاملCan donor splice site recognition occur without the involvement of U1 snRNP?
Many disease-causing mutations affecting donor splice site recognition are reported in the literature. One of the more frequently observed nucleotide changes causing aberrant splicing are due to mutations in the donor splice site which lower the strength of base pairing with U1 snRNA (small nuclear RNA). However, recent data have highlighted the possibility of a recognition mechanism for weak d...
متن کاملExon mutations uncouple 5' splice site selection from U1 snRNA pairing.
It has previously been shown that a mutation of yeast 5' splice junctions at position 5 (GUAUGU) causes aberrant pre-mRNA cleavages near the correct 5' splice site. We show here that the addition of exon mutations to an aberrant cleavage site region transforms it into a functional 5' splice site both in vivo and in vitro. The aberrant mRNAs are translated in vivo. The results suggest that the h...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 1991
ISSN: 0890-9369
DOI: 10.1101/gad.5.8.1430